CSF electrophoresis: an adaptation using cellulose acetate for the identification of oligoclonal banding.
نویسندگان
چکیده
An adaptation of cellulose acetate electrophoresis for studying concentrated cerebrospinal fluid is described. Two hundred and twenty-one patients have been studied, and the specificity for multiple sclerosis and sub-acute sclerosing panencephalitis is discussed. This has been positive for oligoclonal banding (OB) in 79% of patients with clinically definite multiple sclerosis.
منابع مشابه
CSF electrophoresis in one thousand patients.
Agarose and/or cellulose acetate electrophoresis was performed on the CSF of one thousand patients. In patients with clinically definite multiple sclerosis (MS) (N = 267) 92.8% had oligoclonal banding (O.B.). In patients with possible MS (N = 283) O.B. was present in 31.1%. In patients with other neurological diseases (N = 450) O.B. was present in 8% (N = 36). Nineteen non-MS patients with posi...
متن کاملDetection and classification of paraproteins by capillary immunofixation/subtraction.
A selection of 58 specimens with a monoclonal component identified by immunoelectrophoresis and/or immunofixation was analyzed with the immunosubtraction procedure on the Paragon 2000 capillary electrophoresis system. The capillary system detected 93% of the paraproteins and, using immunosubtraction, correctly identified 91% of the paraproteins. Paraproteins that were detected by immunofixation...
متن کاملA comparison of cellulose acetate immunofixation with polyacrylamide gel electrophoresis for the detection of oligoclonal bands in unconcentrated cerebrospinal fluid.
Two methods of electrophoresis for the detection of oligoclonal bands in unconcentrated CSF were compared. A sample of 98 routine CSFs yielded 18 positives by polyacrylamide gel electrophoresis (PAGE) while cellulose acetate electrophoresis with immunofixation (CAIF) gave 13 positives (72% of the PAGE findings). Despite the loss of sensitivity the cellulose acetate electrophoresis was easier to...
متن کاملOligoclonal Bands Are Found in Electrophoretograms of Serum of Patients with Multiple
A recent paper (1) describing oligoclonal banding in CSF indicates that it is necessary to electrophorese paired CSF and serum specimens simultaneously, to differentiate oligoclonal banding caused by intrathecal iinmunoglobulun production from increased permeability of the blood-CSF barrier. This dogma is not correct, as it has been shown by use of polyacrylaimde gel isoelectric focusing and Co...
متن کاملOligoclonal bands are found in electrophoretograms of serum of patients with multiple sclerosis.
A recent paper (1) describing oligoclonal banding in CSF indicates that it is necessary to electrophorese paired CSF and serum specimens simultaneously, to differentiate oligoclonal banding caused by intrathecal iinmunoglobulun production from increased permeability of the blood-CSF barrier. This dogma is not correct, as it has been shown by use of polyacrylaimde gel isoelectric focusing and Co...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Canadian journal of neurological sciences. Le journal canadien des sciences neurologiques
دوره 5 3 شماره
صفحات -
تاریخ انتشار 1978